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chip lysis buffer  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc chip lysis buffer
    Chip Lysis Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+lysis+buffer/pmc13043960-89-4-7
    Average 86 stars, based on 1 article reviews
    chip lysis buffer - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    other:

    Article Title: The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activity.
    Article Snippet: On the second day, the beads were washed twice using low-salt and high-salt solutions and then resuspended in ChIP Elution Buffer (cat#: 7009, CST).

    Chromatin Immunoprecipitation:

    Article Title: FOXA2 promotes metastatic competence in small cell lung cancer.
    Article Snippet: The images were taken using an Oxford Optronix Gelcount instrument and the number of formed clusters was counted. .. Cell lysates in ChIP lysis buffer were sonicated and its supernatant were incubatedwith the antibodies at 4 °Covernight (IgG: Cell Signaling Technology, Cat #: 2729S (1mg/ml) and ASCL1: Cell Signaling Technology, Cat #: 43666S, (100μg/ml). ..

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis.
    Article Snippet: .. Cells were lysed by ChIP lysis buffer (CST), treated with 0.5 μl Micrococcal Nuclease (CST, #10011), and sonicated to yield DNA fragment sizes of 0.2 to 1 kb. .. Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435- 1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator.

    Article Title: RNA Helicase DHX37 Facilitates Liver Cancer Progression by Cooperating with PLRG1 to Drive Superenhancer-Mediated Transcription of Cyclin D1
    Article Snippet: .. The cells were resuspended in ChIP lysis buffer (Cell Signaling Technology; Supplementary Table S3) containing protease inhibitors and/or dithiothreitol for sonication using either a Bioruptor or Covaris sonicator to obtain DNA fragments of 200 to 500 bp. ..

    Article Title: TGFB2-AS1 inhibits triple-negative breast cancer progression via interaction with SMARCA4 and regulating its targets TGFB2 and SOX2
    Article Snippet: Cells were rinsed twice with cold PBS supplemented with 1mM PMSF (Roche) and 1 x protease inhibitor cocktail (CST). .. Cells were then lysed by ChIP lysis buffer (CST) and socinated to shear the chromatin to yield DNA fragment sizes of 0.2 to 1 kb. ..

    Article Title: Myocyte enhancer factor 2a orchestrates vascular redox homeostasis via direct transcriptional activation of SIRT1.
    Article Snippet: .. The cells were harvested, washed twice with ice-cold PBS, and lysed in 1× ChIP lysis buffer supplemented with 1 mM DTT and 1× protease inhibitor cocktail (PIC; CST, Danvers, USA). .. Chromatin was digested with micrococcal nuclease (MNase; CST) at 37°C for 20 min to generate 150–300 bp fragments.

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis
    Article Snippet: .. Cells were lysed by ChIP lysis buffer (CST), treated with 0.5 μl Micrococcal Nuclease (CST, #10011), and sonicated to yield DNA fragment sizes of 0.2 to 1 kb. .. Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435-1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator.

    Article Title: FOXA2 promotes metastatic competence in small cell lung cancer
    Article Snippet: Cells were resuspended with ChIP lysis buffer supplemented with phosphatase inhibitors (Thermo Scientific, Halt Phosphatase Inhibitor Cocktail, Cat #: 78427, 1:1000) and protease inhibitors (Roche cOmplete mini EDTA-free protease inhibitor tablets, Cat #:11836170001) after PBS wash. .. Cell lysates in ChIP lysis buffer were sonicated and its supernatant were incubated with the antibodies at 4 °C overnight (IgG: Cell Signaling Technology, Cat #: 2729S (1 mg/ml) and ASCL1: Cell Signaling Technology, Cat #: 43666S, (100 μg/ml). ..

    Lysis:

    Article Title: FOXA2 promotes metastatic competence in small cell lung cancer.
    Article Snippet: The images were taken using an Oxford Optronix Gelcount instrument and the number of formed clusters was counted. .. Cell lysates in ChIP lysis buffer were sonicated and its supernatant were incubatedwith the antibodies at 4 °Covernight (IgG: Cell Signaling Technology, Cat #: 2729S (1mg/ml) and ASCL1: Cell Signaling Technology, Cat #: 43666S, (100μg/ml). ..

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis.
    Article Snippet: .. Cells were lysed by ChIP lysis buffer (CST), treated with 0.5 μl Micrococcal Nuclease (CST, #10011), and sonicated to yield DNA fragment sizes of 0.2 to 1 kb. .. Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435- 1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator.

    Article Title: RNA Helicase DHX37 Facilitates Liver Cancer Progression by Cooperating with PLRG1 to Drive Superenhancer-Mediated Transcription of Cyclin D1
    Article Snippet: .. The cells were resuspended in ChIP lysis buffer (Cell Signaling Technology; Supplementary Table S3) containing protease inhibitors and/or dithiothreitol for sonication using either a Bioruptor or Covaris sonicator to obtain DNA fragments of 200 to 500 bp. ..

    Article Title: TGFB2-AS1 inhibits triple-negative breast cancer progression via interaction with SMARCA4 and regulating its targets TGFB2 and SOX2
    Article Snippet: Cells were rinsed twice with cold PBS supplemented with 1mM PMSF (Roche) and 1 x protease inhibitor cocktail (CST). .. Cells were then lysed by ChIP lysis buffer (CST) and socinated to shear the chromatin to yield DNA fragment sizes of 0.2 to 1 kb. ..

    Article Title: Myocyte enhancer factor 2a orchestrates vascular redox homeostasis via direct transcriptional activation of SIRT1.
    Article Snippet: .. The cells were harvested, washed twice with ice-cold PBS, and lysed in 1× ChIP lysis buffer supplemented with 1 mM DTT and 1× protease inhibitor cocktail (PIC; CST, Danvers, USA). .. Chromatin was digested with micrococcal nuclease (MNase; CST) at 37°C for 20 min to generate 150–300 bp fragments.

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis
    Article Snippet: .. Cells were lysed by ChIP lysis buffer (CST), treated with 0.5 μl Micrococcal Nuclease (CST, #10011), and sonicated to yield DNA fragment sizes of 0.2 to 1 kb. .. Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435-1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator.

    Article Title: FOXA2 promotes metastatic competence in small cell lung cancer
    Article Snippet: Cells were resuspended with ChIP lysis buffer supplemented with phosphatase inhibitors (Thermo Scientific, Halt Phosphatase Inhibitor Cocktail, Cat #: 78427, 1:1000) and protease inhibitors (Roche cOmplete mini EDTA-free protease inhibitor tablets, Cat #:11836170001) after PBS wash. .. Cell lysates in ChIP lysis buffer were sonicated and its supernatant were incubated with the antibodies at 4 °C overnight (IgG: Cell Signaling Technology, Cat #: 2729S (1 mg/ml) and ASCL1: Cell Signaling Technology, Cat #: 43666S, (100 μg/ml). ..

    Sonication:

    Article Title: FOXA2 promotes metastatic competence in small cell lung cancer.
    Article Snippet: The images were taken using an Oxford Optronix Gelcount instrument and the number of formed clusters was counted. .. Cell lysates in ChIP lysis buffer were sonicated and its supernatant were incubatedwith the antibodies at 4 °Covernight (IgG: Cell Signaling Technology, Cat #: 2729S (1mg/ml) and ASCL1: Cell Signaling Technology, Cat #: 43666S, (100μg/ml). ..

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis.
    Article Snippet: .. Cells were lysed by ChIP lysis buffer (CST), treated with 0.5 μl Micrococcal Nuclease (CST, #10011), and sonicated to yield DNA fragment sizes of 0.2 to 1 kb. .. Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435- 1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator.

    Article Title: RNA Helicase DHX37 Facilitates Liver Cancer Progression by Cooperating with PLRG1 to Drive Superenhancer-Mediated Transcription of Cyclin D1
    Article Snippet: .. The cells were resuspended in ChIP lysis buffer (Cell Signaling Technology; Supplementary Table S3) containing protease inhibitors and/or dithiothreitol for sonication using either a Bioruptor or Covaris sonicator to obtain DNA fragments of 200 to 500 bp. ..

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis
    Article Snippet: .. Cells were lysed by ChIP lysis buffer (CST), treated with 0.5 μl Micrococcal Nuclease (CST, #10011), and sonicated to yield DNA fragment sizes of 0.2 to 1 kb. .. Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435-1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator.

    Article Title: FOXA2 promotes metastatic competence in small cell lung cancer
    Article Snippet: Cells were resuspended with ChIP lysis buffer supplemented with phosphatase inhibitors (Thermo Scientific, Halt Phosphatase Inhibitor Cocktail, Cat #: 78427, 1:1000) and protease inhibitors (Roche cOmplete mini EDTA-free protease inhibitor tablets, Cat #:11836170001) after PBS wash. .. Cell lysates in ChIP lysis buffer were sonicated and its supernatant were incubated with the antibodies at 4 °C overnight (IgG: Cell Signaling Technology, Cat #: 2729S (1 mg/ml) and ASCL1: Cell Signaling Technology, Cat #: 43666S, (100 μg/ml). ..

    Shear:

    Article Title: TGFB2-AS1 inhibits triple-negative breast cancer progression via interaction with SMARCA4 and regulating its targets TGFB2 and SOX2
    Article Snippet: Cells were rinsed twice with cold PBS supplemented with 1mM PMSF (Roche) and 1 x protease inhibitor cocktail (CST). .. Cells were then lysed by ChIP lysis buffer (CST) and socinated to shear the chromatin to yield DNA fragment sizes of 0.2 to 1 kb. ..

    Protease Inhibitor:

    Article Title: Myocyte enhancer factor 2a orchestrates vascular redox homeostasis via direct transcriptional activation of SIRT1.
    Article Snippet: .. The cells were harvested, washed twice with ice-cold PBS, and lysed in 1× ChIP lysis buffer supplemented with 1 mM DTT and 1× protease inhibitor cocktail (PIC; CST, Danvers, USA). .. Chromatin was digested with micrococcal nuclease (MNase; CST) at 37°C for 20 min to generate 150–300 bp fragments.

    Incubation:

    Article Title: FOXA2 promotes metastatic competence in small cell lung cancer
    Article Snippet: Cells were resuspended with ChIP lysis buffer supplemented with phosphatase inhibitors (Thermo Scientific, Halt Phosphatase Inhibitor Cocktail, Cat #: 78427, 1:1000) and protease inhibitors (Roche cOmplete mini EDTA-free protease inhibitor tablets, Cat #:11836170001) after PBS wash. .. Cell lysates in ChIP lysis buffer were sonicated and its supernatant were incubated with the antibodies at 4 °C overnight (IgG: Cell Signaling Technology, Cat #: 2729S (1 mg/ml) and ASCL1: Cell Signaling Technology, Cat #: 43666S, (100 μg/ml). ..



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